Technical Data
D6510-40A
DHX29 (DEAH (Asp-Glu-Ala-His) Box 29, DDX29, FLJ21492, Nucleic acid helicase DDX)
Description:
DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5 untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blotting: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
The epitope recognized maps to a region between residue 50 and 100 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 using the numbering given in entry NP_061903.2 (GeneID 54505).
Purity:
Purified by immunoaffinity chromatography.
Form
Tris-citrate/phosphate buffer, pH 7 to 8
Specificity:
Specific to the human DHX29 protein. Species Crossreactivity: Human. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
General References:
1. de la Cruz, J. et al. (1999) Trends Biochem Sci 24, 192-8, Pisareva, V.P. et al. (2008) Cell 135, 1237-50.