Technical Data
D6510-40C
DHX29 (DEAH (Asp-Glu-Ala-His) Box 29, DDX29, FLJ21492, Nucleic acid helicase DDX)
Description:
DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5 untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide derived from a sequence around Gly63 of human DHX29 protein (KLH-coupled).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.
Specificity:
Recognizes human DHX29 (G63). Species crossreactivity: mouse, rat, monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. de la Cruz, J. et al. (1999) Trends Biochem Sci 24, 192-8, Pisareva, V.P. et al. (2008) Cell 135, 1237-50.