Technical Data
DHX33 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 33, DEAH Box Protein 33, DDX33, DKFZp762F2011, FLJ21972, Putative ATP-dependent RNA Helicase DHX33)
DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Suitable for use in Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Not suitablle for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Immunoprecipitation: 2-5ug/mg
Immunofluorescence (IC): 1:250-1:2000
Immunohistochemistry (paraffin): 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Positive Controls:
Hela Whole Cell Extract

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Recognizes human DHX33. The immunogen recognized maps to a region between aa1-50 of human DHX33 (Asp-Glu-Ala-His) Box Polypeptide (NP_064547.2).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.