		Technical Data
D6510-44A	DHX33 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 33, DEAH Box Protein 33, DDX33, DKFZp762F2011, FLJ21972, Putative ATP-dependent RNA Helicase DHX33)

Description:
DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:2000-1:10,000
Immunoprecipitation: 2-5ug/mg
Optimal dilutions to be determined by the researcher.

Positive Controls:
Hela Whole Cell Extract

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris-citrate/phosphate, pH 7-8, 0.09% sodium azide.

Specificity:

Recognizes human DHX33 at ~79kD. The epitope recognized maps to a region between aa650 and the C-terminus (aa707) of human DHX33 (Asp-Glu-Ala-His) (NP_064547.2).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.