Technical Data
D6510-44A
DHX33 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 33, DEAH Box Protein 33, DDX33, DKFZp762F2011, FLJ21972, Putative ATP-dependent RNA Helicase DHX33)
Description:
DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Applications:
Suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:2000-1:10,000
Immunofluorescence (IC): 1:10-1:2000
Immunoprecipitation: 2-5ug/mg lysate
Optimal dilutions to be determined by the researcher.

Positive Controls:
Hela Whole Cell Extract

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Specificity:
Recognizes human DHX33 at ~79kD. The epitope recognized maps to a region between aa650 and the C-terminus (aa707) of human DHX33 (Asp-Glu-Ala-His) (NP_064547.2). Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Mitoma H, Hanabuchi S, Kim T, et al. The DHX33 RNA Helicase Senses Cytosolic RNA and Activates the NLRP3 Inflammasome. Immunity 2013 Jul 25