Technical Data
D7880-26
2', 3'-Dideoxynucleotide-5'-triphosphate Set (ddNTP) (20mM, 4x1umole, ddATP, ddCTP, ddGTP, ddTTP)
4x50ul
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Technical Data |
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D7880-26 |
2', 3'-Dideoxynucleotide-5'-triphosphate Set (ddNTP) (20mM, 4x1umole, ddATP, ddCTP, ddGTP, ddTTP) |
4x50ul |
| Molecular Biology | Storage: -20°CShipping: Blue Ice |
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2', 3'-ddNTP is a set of 4 synthetic triphosphates lacking of 2'- and 3'- hydroxyl groups, that results in DNA chain termination. Suitable for sequencing DNA by the methods of Sanger and cycle sequence. Applications: Suitable for sequencing DNA by the methods of Sanger and cycle sequence. Concentration: 4x1umol (20mM) |
Quality Control: Functionally tested in cycle sequence with Taq DNA Polymerase™. Purity (HPLC): 95% Lambda Max: ddATP: 261 ddCTP: 281 ddGTP: 253 ddTTP: 267 Extinction Coefficient: ddATP: 15.2x10e3 ddCTP: 9.3x10e3 ddGTP: 13.6x10e3 ddTTP: 9.6x10e3 A250/A260: ddATP: 0.74 ± 0.03 ddCTP: 0.41 ± 0.03 ddGTP: 0.15 ± 0.03 ddTTP: 0.62 ± 0.03 A280/A260: ddATP: 0.17 ± 0.03 ddCTP: 2.41 ± 0.03 ddGTP: 0.67 ± 0.03 ddTTP: 0.81 ± 0.03 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. ™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche. |
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