Technical Data
D7880-26
2', 3'-Dideoxynucleotide-5'-triphosphate Set (ddNTP) (20mM, 4x1umole, ddATP, ddCTP, ddGTP, ddTTP)
4x50ul
Molecular Biology Storage: -20°CShipping: Blue Ice
2', 3'-ddNTP is a set of 4 synthetic triphosphates lacking of 2'- and 3'- hydroxyl groups, that results in DNA chain termination. Suitable for sequencing DNA by the methods of Sanger and cycle sequence.

Applications:
Suitable for sequencing DNA by the methods of Sanger and cycle sequence.

Concentration: 4x1umol (20mM)
Quality Control:
Functionally tested in cycle sequence with Taq DNA Polymerase™.

Purity (HPLC): 95%

Lambda Max:
ddATP: 261
ddCTP: 281
ddGTP: 253
ddTTP: 267

Extinction Coefficient:
ddATP: 15.2x10e3
ddCTP: 9.3x10e3
ddGTP: 13.6x10e3
ddTTP: 9.6x10e3

A250/A260:
ddATP: 0.74 ± 0.03
ddCTP: 0.41 ± 0.03
ddGTP: 0.15 ± 0.03
ddTTP: 0.62 ± 0.03

A280/A260:
ddATP: 0.17 ± 0.03
ddCTP: 2.41 ± 0.03
ddGTP: 0.67 ± 0.03
ddTTP: 0.81 ± 0.03

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.