2', 3'-Dideoxynucleotide-5'-triphosphate Set (ddNTP) (20mM, 4x1umole, ddATP, ddCTP, ddGTP, ddTTP)
|Molecular Biology||Storage: -20°CShipping: Blue Ice|
2', 3'-ddNTP is a set of 4 synthetic triphosphates lacking of 2'- and 3'- hydroxyl groups, that results in DNA chain termination. Suitable for sequencing DNA by the methods of Sanger and cycle sequence.
Suitable for sequencing DNA by the methods of Sanger and cycle sequence.
Concentration: 4x1umol (20mM)
Functionally tested in cycle sequence with Taq DNA Polymerase™.
Purity (HPLC): 95%
ddATP: 0.74 ± 0.03
ddCTP: 0.41 ± 0.03
ddGTP: 0.15 ± 0.03
ddTTP: 0.62 ± 0.03
ddATP: 0.17 ± 0.03
ddCTP: 2.41 ± 0.03
ddGTP: 0.67 ± 0.03
ddTTP: 0.81 ± 0.03
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche.