Technical Data
D8065-31B
Dipeptidyl Peptidase II (DPPII, Quiescent cell proline dipeptidase, QPP, DPP7)
Description:
Dipeptidyl-peptidase II (DPPII), also known as quiescent cell proline dipeptidase (QPP) and
DPP7, shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and
seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases.
They are all prolyl proteases that cleave proteins and peptides after proline residues.
However, DPPII is not a member of the S09 family, but a member of the S28 family that also
includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and
thymus-specific serine peptidase/PRSS16.

Applications:
Suitable for use in ELISA, Neutralization and Western blot. Other applications have not been tested.

Recommended Dilutions:
Neutralization-preincubated with the
enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then
assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by
the antibody at approximately 0.12ug/ml (IC50) under conditions in which the enzyme was
present at 0.1ug/ml and the substrate concentration was 10uM. Considering the molecular
masses of the enzyme (53kD) and the antibody (150kD), IC50 was achieved at
approximately 1:2 molar ratio of the antibody to the enzyme.
Western blot: 1-2ug/ml The detection limit is ~10 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection with
WesternGloTM Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.
Optimal dilutions to be determined by the researcher.

Hybridoma:
mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant human DPPII.

Storage and Stability:

Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a8B15Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRat
Concentration:
~0.5mg/ml
Immunogen:
NS0-derived recombinant human rhDPPII..
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS to a concentration of 0.5mg/ml.
Specificity:
Recognizes human DPPII. Species sequence homology: chimpanzee 99%, mouse 81%, rat 80%, dog 79% and chicken 65%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.