Technical Data
D8065-43D
Diphtheria Toxin, A Subunit (Corynebacterium diphtheriae Toxoid A, DT)
Description:
DT catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kD, it is then processed to form A and B fragments both of which appear to be required for toxicity.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B2750Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Native
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 1% BSA.
Specificity:
Recognizes diphtheria toxin (DT), secreted by certain strains of Corynebacterium diphtheriae. Specific for both the free and bound A subunit of diphtheria toxin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.