Technical Data
D8065-47D
Diphtheria Toxin
Description:
Diphtheria Toxin catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kD, it is then processed to form A and B fragments both of which appear to be required for toxicity.

Applications:
Suitable for use in ELISA, Fluorescence Microscopy, Western Blot, Immunocytochemistry and conjugation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceGoat
Concentration:
~4-5mg/ml
Immunogen:
Purified Diphtheria toxoid.
Purity:
Purified IgG fraction to a purity of ~95%.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Purified diphtheria toxin/toxoid. Demonstrated reactivity with purified toxin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Soria-Guerra, R.E. et al., (2009) Planta 229: 1293-1302.