Technical Data
D8065-48A
Diphtheria Toxin (Anatoxin)
Description:
Diphtheria Toxin catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kD, it is then processed to form A and B fragments both of which appear to be required for toxicity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Recommended Pair:
Capture: D8065-48B
Detection: D8065-48A

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b9B54Affinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Supernatant from C. diphtheriae
Purity:
Purified by Protein G chromatography. >95% pure.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes Diphtheria toxin and anatoxin. Does not react with free A or B subunits of Diphtheria toxin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.