Technical Data
Media
Diploid Medium w/o L-Glutamine (Powder)
D8066
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O264.9
Potassium Chloride400
Sodium Chloride6800
Amino Acids:
L-Arginine•HCl21.06
L-Cystine•2Na•2H2O15.11
L-Histidine•HCl•H2O10.5
L-Isoleucine26.23
L-Leucine26.23
L-Lysine•HCl36.53
L-Methionine7.46
L-Phenylalanine16.51
L-Threonine23.82
L-Tryptophan4.08
L-Tyrosine•Na2•H2O25.15
L-Valine23.43
Vitamins:
Biotin1
Choline Chloride1
Folic Acid1
Nicotinamide1
D-Pantothenic Acid•Ca1
Pyridoxal•HCl1
Riboflavin0.1
Thiamine•HCl1
Other:
D-Glucose1000
Phenol Red, Sodium10
Total:8.7g/L



Directions per Liter: Dissolve 8.7g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.