Technical Data
DMAP1 (DNA Methyltransferase 1-Associated Protein 1, DNMAP1, DNMTAP1, DNMT1-Associated Protein 1, KIAA1425)
DNA methyltransferase 1-associated protein 1 (DMAP1) is involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The protein also specifically potentiatesDXX-mediated repression of glucocorticoid receptor-dependent transcription. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. DMAP1 is found in a wide variety of living organisms from yeasts to humans. The human DMAP1 gene is localized on chromosome 1p34.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-4ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human, mouse and rat brain lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa250-300 of human DMAP1.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Recognizes human DMAP1. Species Crossreactivity: chimpanzee, bovine, canine, mouse, rat. Species sequence homology: zebrafish, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liu, Z. et al. J. Biol. Chem. 279:14120-14128 (2004). 2. Muromoto, RJ. et al. Immunol. 172:2985-2993 (2004).