Technical Data
D8073-02H
DMAP1 (DNA Methyltransferase 1-Associated Protein 1, DNMAP1, DNMTAP1, DNMT1-Associated Protein 1, KIAA1425)
Description:
DNA methylation can help regulate transcriptional silencing via repressive transcription complexes, which include methyl-CpG-binding domain protein and Histone deacetylases (HDACs) DNMT1. This core enzyme for mammalian DNA methylation can also establish a repressive transcription complex consisting of DNMT1, HDAC2 and DMAP1. DMAP1 can interact directly with the transcriptional corepressor TSG101. The DNMT1-DMAP1 exists throughout the S-phase; HDAC2 joind DNMT1 and DMAP1 only during Late S phase. This provides a regulated means to deacetylate heterochromatin following replication.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa438 from mouse DMAP1.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 30% glycerol.
Specificity:
Recognizes mouse DMAP1. Species Crossreactivity: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.