Technical Data
D8075-51C
DMP1 (Dentin Matrix Acidic Phosphoprotein 1, DMP-1, Dentin Matrix Protein 1)
Description:
DMP-1(dentin matrix protein1) is an acidic,100-160kD member of the phosphophoryn family of extracellular matrix proteins. Mouse DMP-1 is found both intra- and extracellularly, where phosphorylated DMP-1 participates in bone mineralization. Secreted mature mouse DMP-1 is a 487aa proteoglycan. It contains multiple serine, glutamic acid, and aspartic acid residues, a GAG attachment site (aa71-72) and an RGD motif (aa350-352). Multiple cleavage sites exist between aas205-253, creating multiple fragments approximately 37kD and 57kD in size. Mature mouse DMP-1 is 87% and 63% aa identical to rat and human DMP-1, respectively.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 2-8ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cells with B cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG111D62Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to human DMP-1 expressed in NSO cells (NP_001073380).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity:
Recognizes human DMP-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.