Technical Data
D8080-01K
DOK1, NT (Docking Protein 1, Downstream Of Tyrosine Kinase 1, p62(dok), pp62)
Description:
DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the N-terminal region of human DOK1 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human DOK1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Liang, X., et al., J. Biol. Chem. 277(16):13732-13738 (2002). 2.Yamakawa, N., et al., EMBO J. 21(7):1684-1694 (2002). 3.Hubert, P., et al., Eur. J. Immunogenet. 27(3):145-148 (2000). 4.Nemorin, J.G., et al., J. Biol. Chem. 275(19):14590-14597 (2000). 5.Nelms, K., et al., Genomics 53(2):243-245 (1998).