		Technical Data
D8080-01K	DOK1, NT (Docking Protein 1, Downstream Of Tyrosine Kinase 1, p62(dok), pp62)

Description:
DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the N-terminal region of human DOK1 (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human DOK1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Liang, X., et al., J. Biol. Chem. 277(16):13732-13738 (2002). 2.Yamakawa, N., et al., EMBO J. 21(7):1684-1694 (2002). 3.Hubert, P., et al., Eur. J. Immunogenet. 27(3):145-148 (2000). 4.Nemorin, J.G., et al., J. Biol. Chem. 275(19):14590-14597 (2000). 5.Nelms, K., et al., Genomics 53(2):243-245 (1998).