Technical Data
D8080-01P
Dok1 (Docking Protein 1, Downstream Of Tyrosine Kinase 1, MGC117395, MGC138860, p62(dok), p62dok, P62DOK, pp62)
Description:
DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:1:500-1:1,000
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human p62Dok around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human p62Dok. Species Cross-Reactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.