		Technical Data
D9010-02	Dopamine Beta Hydroxylase, CT (DBH, Dopamine beta Monooxygenase)

Description:
The production of neurotransmitters is controlled by environmental stimuli, which send signals through second messengers and protein kinase pathways. Regulation of gene expression of dopamine beta hydroxylase and tyrosine hydroxylase, two of the catecholamine biosynthetic enzymes, by cyclic AMP, diacyl glycerol, and Ca2+ leads to increased neurotransmitter biosynthesis. Dopamine beta-hydroxylase catalyzes the conversion of dopamine to noradrenaline and is expressed selectively in noradrenergic and adrenergic neurons in the nervous system. The disruption of the DBH gene is thought to cause changes in development and behavior in certain models. Both DBH and the multiple regulatory pathways of DBH are of increasing research in many neurological diseases.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000; Recognizes single (or double) MW ~70-75000 band(s) in SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE
Immunohistochemistry: Evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
30ug		-20°C	Blue Ice	Human	Sheep

Concentration:

~0.1mg/ml

Immunogen:

Peptide corresponding to the C-terminal region of human Dopamine Beta Hydroxylase. Cellular Localization: exists in soluble form (in chromaffin granules) and as membrane-bound.

Purity:

Purified

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Specificity:

Recognizes human Dopamine Beta Hydroxylase, CT. Species Crossreactivity: monkey, mouse. Does not recognize sheep, canine or rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Swanson, D.J., et al. J Biol Chem. 273 (37): 24065-74, 1998. 2. Kim, H.S., et al. J Neurochem. 71(1): 41-50, 1998. 3. Thomas, S.A., and R.D. Palmiter. Adv Pharmacol. 42: 57-60, 1998. 4. Sabban, E.L., and B.B. Nankova. Adv Pharmacol. 42: 53-56, 1998.