Technical Data
D9010-03
Dopamine Beta Hydroxylase (DBH, Dopamine beta Monooxygenase)
Description:
Dopamine-beta--hydroxylase (DBH) is the enzyme responsible for conversion of dopamine to noradrenaline. Tissue specificity consists of chromaffin granules of the adrenal medulla and synaptic vesicles of the sympathetic nervous system. It exists as both, a soluble and membrane-bound form in chromaffin granules.

Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 1:5000-1:10,000
Immunohistochemistry (Frozen): 1:20,000-1:50,000
Western Blot: 1:5000-1:10,000 (ECL)
Optimal dilutions to be determined by the reasearcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
250ul-20CBlue IceRatSheep
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to aa43-62, SEPPESPFPYHIPLDPEGTL of the N-terminal rat DBH (dopamine-beta--hydroxylase).
Purity:
Serum
Form
Suoplied as a liquid, 0.2% sodium azide.
Specificity:
Recognizes rat DBH. Specifically stains chromaffin cells of the adrenal medulla and DBH-containing cells of the sympathetic nervous system, including locus coeruleus and medulla oblongata. Species Sequence Homology: >85% in human, bovine and mouse DBH.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.