Technical Data
D9013-04
Dopa Decarboxylase (DOPA)
Description:
DDC catalyses the second reaction in the biosynthesis of catecholamines and serotonin. In the final stages of dopamine synthesis, this enzyme converts L-3,4-dihydroxyphenylalanine (L-DOPA) to dopamine. This pathway is of particular research interest since L-DOPA is regularly used to treat the symptoms of Parkinson’s disease.

Applications:
Suitable for use in Western Blot and Immunohistochemistry (frozen). Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000, detects a band of approximately 55kD in bovine adrenal medulla lysates.
Optimal dilution determined by the researcher

Recommended Secondary Reagents:
Sheep anti rabbit IgG:HRP

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the N-terminal region of human DOPA Decarboxylase.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 0.01% BSA, 0.09%sodium azide, 50% Glycerol.
Specificity:
Specific for DOPA decarboxylase (DDC), also known as aromatic L-amino acid decarboxylase (AACS). Species Crossreactivity: Bovine, canine, ovine and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Banik, U. et al. (1997). Interaction of Phosphorylated Tryptophan Hydroxylase with 14-3-3 Proteins. J. Biol. Chem. 272: 26219 - 26225.