DOPA Decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).
Suitable for use in Western Blot. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|SDS denatured recombinant bovine DOPA Decarboxylase (DDC) expressed in E. coli and purified from inclusion bodies.|
|Purified by immunoaffinity chromatography.|
|10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg per ml BSA and 50% glycerol|
|The affinity-purified antibody has been tested using blot immunolabeling procedures, in which a single MW ~55kDa band is detected. Species Crossreactivity: This antibody recognizes DDC in bovine, canine, guinea pig, human, ,porcine, rat and sheep adrenal medulla and caudate.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Berry MD, Juorio AV, Li XM, Boulton AA (1996) Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem Res 21:1075-1087.|
2. Haycock JW, Becker L, Ang L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585.
3. Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
4. Waymire JC, Haycock JW (2002) Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells. J Neurochem 81:589-593.
5. Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
6. Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.