Technical Data
D9014-02
Dopamine Receptor, D1a
Description:
Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long (D2L) and short (D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:250-1:500. Unboiled mouse brain lysate using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b2Q48Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant rat D1a Receptor, carboxy terminus.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes rat Dopamine Receptor D1a. Species Crossreactivity: Mouse. Does not crossreact with human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Luedtke, R., et al., Immunoblot and immunohistochemical comparison of mouse monoclonal antibodies for the rat D1a and D1b dopamine receptor subtypes. Journal of Immunology 101: 170-187 (1999).