Technical Data
D9016-10
Dopamine Receptor, D2L, D2S
Description:
The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Two isoforms are reported for the D2 Dopamine Receptor, isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 0.5-1.0ug/ml
Western Blot: 1:1000. Detected Dopamine Receptor D2 on 10ug of human brain lysates.
Immunoprecipitation: 1-10ug/ml. Use 100-400ug of protein. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms.
Immunohistochemistry (Paraformaldehyde fixed sections): 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Monkey brain tissue, rat or mouse brain lysate

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to the cytoplasmic loop #3 (28aa) of the human D2 receptor (NM_000795.2).
Purity:
Purified by immunoaffinity chromotography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity:
Recognizes human Dopamine Receptor D2 short and long form at ~50kD. No significant homology with other dopamine receptors (D1 and D3-D5). Species Crosseactivity: monkey, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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