Technical Data
D9150-78
DPM1, ID (Dolichol-phosphate Mannosyltransferase, Dolichol-phosphate Mannose Synthase, DPM Synthase, Dolichyl-phosphate beta-D-mannosyltransferase, Mannose-P-dolichol Synthase, MPD Synthase)
|
Technical Data |
|
D9150-78 |
DPM1, ID (Dolichol-phosphate Mannosyltransferase, Dolichol-phosphate Mannose Synthase, DPM Synthase, Dolichyl-phosphate beta-D-mannosyltransferase, Mannose-P-dolichol Synthase, MPD Synthase) |
|
|
Description: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||