Technical Data
D9150-78B
DPM1 (Dolichol-phosphate Mannosyltransferase, Dolichol-phosphate Mannose Synthase, DPM Synthase, Dolichyl-phosphate beta-D-mannosyltransferase, Mannose-P-dolichol Synthase, MPD Synthase)
Description:
Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.1-0.3ug/ml, observed in human liver lysates on ~28kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to RELEVRSPRQNKYS-C, from human DPM1, at N-terminal (NP_003850.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human DPM1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ashida H, Maeda Y, Kinoshita T DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3 J Biol Chem. 2006 Jan 13;281(2):896-904.