Technical Data
D9150-79
DPM1 (Dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit, RP23-391M18.1, AI118379, AI194292, OTTMUSP00000017164, dolichol monophosphate mannose synthase, dolichyl-phosphate mannosyltransferase polypeptide 1)
Description:
Dolichol-phosphate mannose (DPM1) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Defective surface expression of GPI-anchored proteins occurs when there is a lack of DPM1. DPM1 is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to PQGRSSRQDKYS-C from the N-Terminus of rat DPM1 (NP_034202.1).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes rat DPM1. Sequence Homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ashida H, Maeda Y, Kinoshita T. DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3. J Biol Chem. 2006 Jan 13;281(2):896-904. PMID: 16280320.