		Technical Data
D9475	DRIP-130 (Vitamin D Receptor Interacting Protein 130, Sur2, CRSP130, ARC130, NAT140, TRAP150b)

Description:
DRIPs are a distinct set of ligand-dependent proteins that interact with the vitamin D receptor (VDR). Together, these proteins constitute a new cofactor complex. DRIPs bind to several nuclear receptors to mediate ligand-dependent enhancement of transcription by VDR and the thyroid-hormone receptor in cell-free transcription assays. The DRIPs are almost indistinguishable from components of another cofactor complex called ARC, which is recruited by other types of transcription activators to mediate transactivation on chromatin-assembled templates. The role of nuclear-receptor ligands may, in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes. In humans, interaction with Sur-2 is required for transcription to be activated by the activation domain of a transcription factor of the ETS-family in response to activated mitogen-activated protein (MAP) kinase.

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Detects a 130kD band
Immunoprecipitation: 1:100-1:200. Pre-clearing of the lysate with a non-specific rabbit IgG is helpful to reduce background.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ul		4°C (-20°C Glycerol)	Blue Ice	Mouse	Rabbit

Concentration:

~80mg/ml

Immunogen:

Synthetic peptide corresponding to mouse DRIP-130 residues 897-916 (based on human DRIP-130 residue numbering) conjugated to KLH.

Purity:

Purified serum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.

Specificity:

Recognizes mouse DRIP-130 (vitamin-D-receptor interacting protein 130). Used to detect DRIP130 using nuclear extracts from mouse thymus, spleen and brain. Species Crossreactivity: Human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Boyer, T.G., et al., Nature 399(6733): 276-279 (1999). 2. Rachez, C., et al., Nature 398(6730): 824-828 (1999). 3. Ko, L., et al., Proc. Natl. Acad. Sci. USA 97(11): 6212-6217 (2000). 4. Chiba, N., et al., J. Biol. Chem. 275(15): 10,719-10,722 (2000). 5. Hittelman, A.B., et al., EMBO J. 18(19): 5380-5388 (1999).