Technical Data
DRIP-130 (Vitamin D Receptor Interacting Protein 130, Sur2, CRSP130, ARC130, NAT140, TRAP150b)
DRIPs are a distinct set of ligand-dependent proteins that interact with the vitamin D receptor (VDR). Together, these proteins constitute a new cofactor complex. DRIPs bind to several nuclear receptors to mediate ligand-dependent enhancement of transcription by VDR and the thyroid-hormone receptor in cell-free transcription assays. The DRIPs are almost indistinguishable from components of another cofactor complex called ARC, which is recruited by other types of transcription activators to mediate transactivation on chromatin-assembled templates. The role of nuclear-receptor ligands may, in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes. In humans, interaction with Sur-2 is required for transcription to be activated by the activation domain of a transcription factor of the ETS-family in response to activated mitogen-activated protein (MAP) kinase.

Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Detects a 130kD band
Immunoprecipitation: 1:100-1:200. Pre-clearing of the lysate with a non-specific rabbit IgG is helpful to reduce background.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul4C/-20CBlue IceMouseRabbit
Synthetic peptide corresponding to mouse DRIP-130 residues 897-916 (based on human DRIP-130 residue numbering) conjugated to KLH.
Purified serum
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
Recognizes mouse DRIP-130 (vitamin-D-receptor interacting protein 130). Used to detect DRIP130 using nuclear extracts from mouse thymus, spleen and brain. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Boyer, T.G., et al., Nature 399(6733): 276-279 (1999). 2. Rachez, C., et al., Nature 398(6730): 824-828 (1999). 3. Ko, L., et al., Proc. Natl. Acad. Sci. USA 97(11): 6212-6217 (2000). 4. Chiba, N., et al., J. Biol. Chem. 275(15): 10,719-10,722 (2000). 5. Hittelman, A.B., et al., EMBO J. 18(19): 5380-5388 (1999).