Drop-out Base With Raffinose (DO, Dropout, Powder)
Molecular Biology Grade
Storage RT/4°C Shipping RT
Components shown as g/liter
Nitrogen, carbon, trace element, and vitamin source for yeast growth. Supplement with the appropriate DO Mix for selection and propagation of yeast transformants. Drop-out Base With Raffinose is glucose and galactose free for the reversible repression of galactose inducible genes that is relieved by the addition of galactose to 4% or the resuspension of cells in galactose media.
All lots are quality control tested using the appropriate yeast strains and transformants.
• Convenient Powders
• Useful for One-Hybrid and Two-Hybrid Systems
• Competitively Priced...!
BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries
Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates
Homogeneous: Proprietary blending technology ensures complete mixing of components
Directions per Liter: Dissolve 26.7g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Appearance: White, homogeneous, free flowing powder
Solubility: Colorless, clear, complete
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kaiser, C., Michaelis, S., Mitchell, A., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, Appendix A: 210, CSHL Press (1994).