Technical Data
Media
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Technical Data
Media
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Drop-out Base With Galactose (Powder)
D9502
Molecular Biology Grade
Storage 4°C Shipping RT
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Components shown as g/liter
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Nitrogen, carbon, trace element, and vitamin source for yeast growth. Supplement with the appropriate DO Mix for selection and propagation of yeast transformants. Drop-out Base With Galactose is specially formulated to be glucose free for the rapid induction of galactose-inducible genes. All lots are quality control tested using the appropriate yeast strains and transformants. • Convenient Powders • Useful for One-Hybrid and Two-Hybrid Systems • Competitively Priced...! BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Directions per Liter: Dissolve 26.7g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time. Appearance: White, homogeneous, free flowing powder Solubility: Colorless, clear, complete pH 4.0-5.0: Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Kaiser, C., Michaelis, S., Mitchell, A., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, Appendix A: 210, CSHL Press (1994). |