Drop-out Base With Raffinose and Agar (DO, Dropout, Powder)
Molecular Biology Grade
Storage RT Shipping RT
Components shown as g/liter
Nitrogen, carbon, trace element, and vitamin source for yeast growth. Supplement with the appropriate DO Mix for selection and propagation of yeast transformants. Drop-out Base With Raffinose and Agar is glucose and galactose free for the reversible repression of galactose-inducible genes. Galactose-inducible genes can be activated by replica plating to galactose plates.
All lots are quality control tested using the appropriate yeast strains and transformants.
Useful for One-Hybrid and Two-Hybrid Systems
BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries
Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates
Homogeneous: Proprietary blending technology ensures complete mixing of components
Directions per Liter: Dissolve 43.7 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ēC (15psi).
Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Appearance: White to off-white, homogeneous, free flowing powder
Solubility: Colorless, clear, complete
pH As reported:
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kaiser, C., Michaelis, S., Mitchell, A., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, Appendix A: 210, CSHL Press (1994).