Technical Data
Media
Drop-out Mix Synthetic Minus Adenine, Histidine, Leucine, Tryptophan, Uracil w/o Yeast Nitrogen Base (DO, Dropout, Powder)
D9540-05
Molecular Biology Grade
Storage RT/4°C    Shipping RT
Formulation:
Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus adenine, histidine, leucine, tryptophan and uracil:
Amino Acids:
p-Aminobenzoic acid0.2g
Alanine2.0g
Arginine2.0g
Asparagine2.0g
Aspartic acid2.0g
Cysteine2.0g
Glutamic acid2.0g
Glutamine2.0g
Glycine2.0g
Isoleucine2.0g
Lysine2.0g
Methionine2.0g
Phenylalanine2.0g
Proline2.0g
Serine2.0g
Threonine2.0g
Tyrosine2.0g
Valine2.0g
Nucleotide Bases:
Inositol2.0g
Use 1.37g/liter
100g makes ~50 liters
Drop-out Mix Synthetic Minus Adenine, Histidine, Leucine, Tryptophan, Uracil w/o Yeast Nitrogen Base is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics.

All lots are quality control tested using the appropriate yeast strains and transformants.

• Convenient Powders
• Useful for One-Hybrid and Two-Hybrid Systems
• Competitively Priced...!

Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates

Homogeneous: Proprietary blending technology ensures complete mixing of components

Companion Products:
D9500: Drop-out Base With Glucose (Powder)
D9501: Drop-out Base With Raffinose (Powder)
D9502: Drop-out Base With Galactose (Powder)
D9510: Drop-out Base With Glucose and Agar (Powder)
D9511: Drop-out Base With Raffinose and Agar (Powder)
D9512: Drop-out Base With Galactose and Agar (Powder)
Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)
Y2025: Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder)
Y2030: Yeast Nitrogen Base w/o AA, Carbohydrate & w/o AS (YNB) (Powder)
Y2035: Yeast Nitrogen Base w/AA & w/AS, Vitamin Free (YNB) (Powder)
Y2035-05: Yeast Nitrogen Base (YNB) w/AA & w/AS, Vitamin Free, w/o Dextrose (Powder)
Y2036: Yeast Nitrogen Base w/o AA, AS, Thiamine HCl (Powder)
Y2037: Yeast Nitrogen Base w/o AA & w/AS (YNB) and w/o Fe, Zn, Mn, Cu (Powder)


Directions per Liter (Broth): Mix 1.37 grams of drop-out mix with 26.7g Y2025 Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS or 6.7g Y2020 Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate drop-out base per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. 

Directions per Liter (Plates): Mix 1.37 grams of drop-out mix with 43.7g D9510-01 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers,  loosen caps and autoclave for 15 minutes at 121ºC (15psi).Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White, homogeneous, free flowing powder

Solubility: Colorless, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Kaiser, C., Michaelis, S., Mitchell, A., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, Appendix A: 210, CSHL Press (1994).