Technical Data
Media
Drop-out Mix Minus Histidine, Leucine, Lysine, Tryptophan, Uracil w/o Yeast Nitrogen Base (DO, Dropout, Powder)
D9543-01
Molecular Biology Grade
Storage RT/4C    Shipping RT
Formulation:
Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases Minus Histidine, Leucine, Lysine, Tryptophan, Uracil:
Amino Acids:
Alanine2.0g
Arginine2.0g
Asparagine2.0g
Aspartic acid2.0g
Cysteine2.0g
Glutamic acid2.0g
Glutamine2.0g
Glycine2.0g
HistidineAbsent
LeucineAbsent
Isoleucine2.0g
LysineAbsent
Methionine2.0g
Phenylalanine2.0g
Proline2.0g
Serine2.0g
Threonine2.0g
TryptophanAbsent
Tyrosine2.0g
Valine2.0g
Nucleotide Bases:
Adenine0.5g
UracilAbsent
Vitamins:
p-Aminobenzoic acid0.2g
Inositol2.0g
Use 1.32g/liter
100g makes ~50 liters
Drop-out Mix Minus Histidine, Leucine, Lysine, Tryptophan, Uracil w/o Yeast Nitrogen Base is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics.

All lots are quality control tested using the appropriate yeast strains and transformants.

Convenient Powders
Useful for One-Hybrid and Two-Hybrid Systems
Competitively Priced...!

Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates

Homogeneous: Proprietary blending technology ensures complete mixing of components

Companion Products:
D9500: Drop-out Base With Glucose
D9501: Drop-out Base With Raffinose
D9502: Drop-out Base With Galactose
D9510: Drop-out Base With Glucose and Agar
D9511: Drop-out Base With Raffinose and Agar
D9512: Drop-out Base With Galactose and Agar
Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB)
Y2025: Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB)
Y2030: Yeast Nitrogen Base w/o AA, Carbohydrate & w/o AS (YNB)
Y2035: Yeast Nitrogen Base w/AA & w/AS, Vitamin Free (YNB)
Y2035-05: Yeast Nitrogen Base (YNB) w/AA & w/AS, Vitamin Free, w/o Dextrose
Y2036: Yeast Nitrogen Base w/o AA, AS, Thiamine HCl
Y2037: Yeast Nitrogen Base w/o AA & w/AS (YNB) and w/o Fe, Zn, Mn, Cu


Directions per Liter: Mix 1.32g of drop-out mix with 26.7g drop-out base w/ carbohydrate (YNB) (or 6.7g drop-out base w/o carbohydrate) (or 43.7g drop-out base w/ agar) per liter of distilled/deionized water. Heat with stirring until completely solubilized. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: White, homogeneous, free flowing powder

Solubility: Colorless, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Kaiser, C., Michaelis, S., Mitchell, A., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, Appendix A: 210, CSHL Press (1994).