Technical Data
Media
Drosophila D-22 Insect Medium (Powder)
D9600
Molecular Biology Grade
Storage RT/4°C    Shipping RT
Components shown as g/L
Inorganic Salts:
Calcium Chloride0.8
Magnesium Chloride0.421654
Magnesium Sulfate1.641152
Sodium Acetate0.013868
Sodium Phosphate Monobasic0.330769
Amino Acids:
L-Glutamic acid, Potassium4.975
L-Glutamic acid, Sodium7.98
Glycine5.0
Vitamins:
p-Aminobenzoic acid0.00002
D-Biotin0.00001
Choline Chloride0.0002
Folic acid0.00002
myo-Inositol0.00002
Niacin0.00002
D-Pantothenic acid •Ca0.00002
Pyridoxine•HCl0.00002
Riboflavin0.00002
Thiamine•HCl0.00002
Other:
D+Glucose1.8
Lactalbumin Hydrolysate13.6
L-Malic acid0.6
Succinic acid, Na2•6H200.055
Yeast Extract1.36
Total38.58g/L
Used to promote growth of primary and established Drosophila melanogaster cells, including cell line K. Supplement with fetal bovine serum (5-20%).


Directions per Liter: Dissolve 38.58g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light tan to tan, homogeneous, free flowing powder

Solubility: Yellow, clear, complete

pH: 4.8±1.5


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Siller, K.H. et al., (2005) Mol Biol Cell Vol. 16:5127-5140. 1. Echalier, G., Ohannessian, A., In Vitro 6: 162-172 (1970).