Technical Data
Media
Drosophila Diet Medium K12, High Efficiency, (Powder) (Fruitfly diet)
D9600-07B
Microbiological Grade
Storage RT/4C    Shipping RT

Used for the maintenance of Drosophila melanogaster and other insects.
K12 Medium is a new insect food particularly indicated for Drosophila melanogaster flies growth and propagation. Fruit flies are maintained as live culture by transferring adults to fresh medium every few generations. Therefore, good quality food yielding high numbers of healthy flies is critical for stock maintenance. Our medium has many advantages over existing rich traditional media (containing molasses or corn)

K12 Medium yields about 200% more larvae and adult flies (Fig.1). This is particularly relevant for the propagation of precious mutant Drosophila strains, which are less viable, used, for example, as models for studying human genetic diseases;

K12 Medium increases the levels of egg deposition (18-20 hours) of about 50% (Fig.2)

K12 Medium greatly improves (about 100%) the overall hatching rate (18-20 hours) (flies raised on K12 Medium hatch over a 4-5 day period instead of the 2-3 days of traditional media), greatly facilitating the collection of virgins and the use of young adults for healthy crosses (Fig.3);

K12 Medium is resistant to microbial contamination and to liquification. This latter feature is
particularly relevant for successful maintenance of sick stocks, quick expansion of stocks
and for mass culture of larvae and adults;

K12 Medium is on average 60% cheaper than other rich traditional media (see Table 1);

K12 Medium is very easy to prepare, store and handle. It comes as a premixed powder to be
reconstituted in water.

Source of K12 Medium:
After nutritional and developmental studies conducted in several wild type and mutant stocks of D. melanogaster, we developed a medium containing the same nutritional sources found in commonly used fly food plus K12, an additional natural ingredient that improves fly development by increasing the number of healthy hatching adults per cross. K12 is extracted from natural sources, currently used in human and animal food industry, and is neither toxic nor cancerous.


Directions per Liter (100ml): Dissolve19.85 grams in 100ml of ddH2O, heat and boil for 15 min while stirring. Add 1ml ethanol to T2300. Add to the boiled solution upon cooling. Dispense into appropriate container. Store at 4C.

Directions per Liter (1L): Dissolve 198.5 grams in 1L of ddH2O, heat and boil for 15 min while stirring. Add 10ml ethanol to T2300. Add to the boiled solution upon cooling. Dispense into appropriate container. Store at 4C.Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Brown, homogeneous, free flowing powder

Directions per Liter (100ml): Dissolve19.85 grams in 100ml of ddH2O, heat and boil for 15 min while stirring. Add 1ml ethanol to T2300. Add to the boiled solution upon cooling. Dispense into appropriate container. Store at 4C.

Directions per Liter (1L): Dissolve 198.5 grams in 1L of ddH2O, heat and boil for 15 min while stirring. Add 10ml ethanol to T2300. Add to the boiled solution upon cooling. Dispense into appropriate container. Store at 4C.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time. Important Note


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.