Technical Data
D9641-01B
DVL3 (Dishevelled-3, Dsh Homolog 3, Segment Polarity Protein Dishevelled Homolog DVL-3, KIAA0208)
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Technical Data |
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D9641-01B |
DVL3 (Dishevelled-3, Dsh Homolog 3, Segment Polarity Protein Dishevelled Homolog DVL-3, KIAA0208) |
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Description: The human DVL-3 gene encodes a predicted 716 amino acid protein which exhibits 98% amino acid identity with mouse Dsh-3 and 49% with Drosophila Dsh. Dsh-3 was mapped to 3q27. The expression of Dsh-3 mRNA was detected in 30 human cell lines and 2 primary cell cultures. Dsh-3 mRNA was detected in normal human breast tissues and tumors. Statistically, there was no difference in Dsh-3 mRNA level between normal breast tissues and tumors. In human colorectal samples, Dsh-3 was expressed equally in matched normal tissues, polyps and tumors. The data indicates that Dsh-3 is widely expressed in human cells and may have a widespread role in signal transduction (1). Results indicate that the human dishevelled genes constitute a multigene family and that Dsh 3 proteins are highly conserved among metazoans. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1000 Immunohistochemistry: 1:100-250 Immunocytochemistry: 1:100-250 Flow Cytometry: 1:30 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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