Technical Data
DVL3 (Dishevelled-3, Dsh Homolog 3, Segment Polarity Protein Dishevelled Homolog DVL-3, KIAA0208)
The human DVL-3 gene encodes a predicted 716 amino acid protein which exhibits 98% amino acid identity with mouse Dsh-3 and 49% with Drosophila Dsh. Dsh-3 was mapped to 3q27. The expression of Dsh-3 mRNA was detected in 30 human cell lines and 2 primary cell
cultures. Dsh-3 mRNA was detected in normal human breast tissues and tumors. Statistically, there was no difference in Dsh-3 mRNA level between normal breast tissues and tumors. In human colorectal samples, Dsh-3 was expressed equally in matched normal tissues, polyps and tumors. The data indicates that Dsh-3 is widely expressed in human cells and may have a widespread role in signal transduction (1). Results indicate that the human dishevelled genes constitute a multigene family and that Dsh 3 proteins are highly conserved among metazoans.

Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1000
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:100-250
Flow Cytometry: 1:30
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A synthetic peptide corresponding to residues near the C-terminus of human Dsh-3
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Recognizes human Dsh3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bui TD, et al. Biochem Biophys Res Commun 239(2): 510-6, 1997. 2. Semenov MV, et al. Genomics 42(2):302-10, 1997