Technical Data
D9650
Ductin (Vha16, EP2372, V-ATPase, VHA, Vha1, vha16, vha16-1, VHA16K, Vhac)
Description:
Ductin is the major core component of gap junctions and recent structural data shows it to be a four alpha-helical bundle which fits particularly well into a low resolution model of the gap junction channel. Ductin is also the main membrane component of the vacuolar H+-ATPase that is found in all eukaryotes and it seems likely that the gap junction channel first evolved as a housing for the rotating spindle of these proton pumps. Because ductin protrudes little from the membrane, other proteins are required to bring cell surfaces close enough together to form gap junctions. Such proteins may include connexins, a large family of proteins found in vertebrates.

Applications:
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:1000
Immunocytochemistry: 1:100-1:500
Immunohistochemistry: 1:100-1:500 on frog and 1:100-1:2000 on chicken.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to human Ductin (100% conserved in all species).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 50% glycerol. No preservative added.
Specificity:
Recognizes human ductin (the 16kD or c subunit of the V-ATPase). Has been specifically tested on flatworm (invertebrate), frog (vertebrate) and chick embryo tissue.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.