Technical Data
Media
Dulbecco’s MEM (DMEM) w/o Glucose, Methionine, Cystine, Histidine (Powder)
D9800-02A
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/Liter
Inorganic Salts:
Calcium Chloride, anhy0.200
Ferric Nitrate•9H2O0.0001
Magnesium Sulfate0.09767
Potassium Chloride0.4
Sodium Chloride6.4
Sodium Phosphate Monobasic•H2O0.125
Amino Acids:
L-Arginine•HCl0.084
L-CystineAbsent
L-Glutamine0.584
Glycine0.03
L-Histidine•HCl•H2OAbsent
L-Isoleucine0.105
L-Leucine0.105
L-Lysine•HCl0.146
L-MethionineAbsent
L-Phenylalanine0.066
L-Serine0.042
L-Threonine0.095
L-Tryptophan0.016
L-Tyrosine•2Na•2H2O0.104
L-Valine0.094
Vitamins:
Choline Chloride0.004
Folic Acid0.004
i-Inositol0.0072
Niacinamide0.004
D-Pantothenic Acid•Ca0.004
Pyridoxal•HCl0.004
Riboflavin0.0004
Thiamine•HCl0.004
Other:
D-GlucoseAbsent
Phenol Red, Sodium0.015
Pyruvic Acid, Sodium0.110
Total:8.85g/Liter
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. L-Glutamine and Glucose are absent. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 8.85g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Orange, homogenous, free flowing

Solubility: Orange, clear, complete

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).