Technical Data
Media
Dulbecco’s MEM (DMEM) Low Glucose, w/o Phenol Red, Folic Acid, Niacinamide, Pyridoxal, Riboflavin, Thiamine (Powder)
D9800-12
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/Liter
Inorganic Salts:
Calcium Chloride•2H2O0.265
Ferric Nitrate•9H2O0.0001
Magnesium Sulfate0.09767
Potassium Chloride0.4
Sodium Chloride6.4
Sodium Phosphate Monobasic0.109
Amino Acids:
L-Arginine•HCl0.084
L-Cystine•2HCl0.0626
L-Glutamine0.584
Glycine0.03
L-Histidine•HCl•H2O0.042
L-Isoleucine0.105
L-Leucine0.105
L-Lysine•HCl0.146
L-Methionine0.03
L-Phenylalanine0.066
L-Serine0.042
L-Threonine0.095
L-Tryptophan0.016
L-Tyrosine•2Na•2H2O0.10379
L-Valine0.094
Vitamins:
Choline Chloride0.004
Folic AcidAbsent
myo-Inositol0.0072
NiacinamideAbsent
D-Pantothenic Acid•Ca0.004
Pyridoxal•HClAbsent
RiboflavinAbsent
Thiamine•HClAbsent
Other:
D-Glucose1
Phenol Red, SodiumAbsent
Pyruvic Acid, Sodium0.11
Total:10.00g/Liter
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 10g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White, homogeneous, free-flowing powder

Solubility: Colorless, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.