Technical Data
Media
Dulbecco’s MEM (DMEM) Low Glucose, w/o Amino Acids, Pyruvic Acid (Powder)
D9800-13
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/Liter
Inorganic Salts:
Calcium Chloride•2H2O 0.265
Ferric Nitrate•9H2O 0.0001
Magnesium Sulfate 0.09767
Potassium Chloride 0.4
Sodium Chloride 6.4
Sodium Phosphate Monobasic 0.109
Amino Acids:
L-Arginine•HClAbsent
L-Cystine•2HClAbsent
L-GlutamineAbsent
GlycineAbsent
L-Histidine•HCl•H2OAbsent
L-IsoleucineAbsent
L-LeucineAbsent
L-Lysine•HClAbsent
L-MethionineAbsent
L-PhenylalanineAbsent
L-SerineAbsent
L-ThreonineAbsent
L-TryptophanAbsent
L-Tyrosine•2Na•2H2OAbsent
L-ValineAbsent
Vitamins:
Choline Chloride0.004
Folic Acid0.004
myo-Inositol0.0072
Niacinamide0.004
D-Pantothenic Acid•Ca0.004
Pyridoxal•HCl0.004
Riboflavin0.0004
Thiamine•HCl0.004
Other:
D-Glucose1
Phenol Red, Sodium0.0159
Pyruvic Acid, SodiumAbsent
Total:8.32g/Liter
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 8.32g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light tan to orange, homogeneous, free-flowing powder

Solubility: Yellow to orange, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.