Technical Data
Dulbecco’s MEM (DMEM) Vitamin Mix 25x, w/o Thiamine
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Choline Chloride0.100
Folic Acid0.100
D-Pantothenic Acid-Ca0.100
Pyroxidal HCl0.100
Thiamine HClAbsent
Total 0.690g/L
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Directions per Liter: Dissolve 0.690g in 800-900ml of ddH2O stirring gently until completely solubilized. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Yellow, homogenous, free flowing powder

Solubility (1X): Colorless to yellow, clear, complete

pH (1X): As Reported

Endotoxin: 1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R. and Freeman, G. Virology. 8, 396-397. (1959). 2. Smith, J.D., Freeman,G., Vogt, M. and Dulbecco, R. The Nucleic Acid of Polyoma Virus. 12, 185-196. (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89. (1970). 4. Rutzky, L.P. and Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro. 9, 468. (1974).