Technical Data
Media
Dulbecco’s MEM (DMEM) High Glucose, 10X, w/Pyridoxal•HCl w/o L-Glutamine (Powder)
D9802-05A
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O2.649
Ferric Nitrate•9H2O0.001
Magnesium Sulfate0.977
Potassium Chloride4.00
Sodium Chloride64.00
Sodium Phosphate Mono•H2O1.25
Amino Acids:
L-Arginine•HCl0.084
L-Cystine0.480
L-Glutamine Absent
Glycine0.30
L-Histidine•HCl•H2O0.042
L-Isoleucine1.048
L-Leucine1.048
L-Lysine•HCl1.462
L-Methionine0.30
L-Phenylalanine0.66
L-Serine0.42
L-Threonine0.952
L-Tryptophan0.16
L-Tyrosine0.72
L-Valine0.936
Vitamins:
Choline Chloride0.04
Folic Acid0.04
i-Inositol0.07
Nicotinamide0.04
D-Pantothenic Acid, Ca0.04
Pyridoxal•HCl0.04
Riboflavin0.004
Thiamine•HCl0.04
Other:
D-Glucose45.00
Phenol Red, Sodium0.15
Pyruvic Acid, Sodium1.10
Total: 128.05g/L (10X)
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 128.05g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 37.5g sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white to light orange, homogenous, free flowing powder

Solubility (10X): Yellow to orange, clear, complete. Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization.

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).