Technical Data
Media
Dulbecco’s MEM (DMEM) High Glucose, 1X, w/Pyridoxal•HCl w/o L-Glutamine, Calcium Chloride (Powder)
D9802-05B
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2OAbsent
Ferric Nitrate•9H2O0.0001
Magnesium Sulfate0.0977
Potassium Chloride0.4
Sodium Chloride6.4
Sodium Phosphate Monobasic
Monohydrate0.125
Amino Acids:
L-Arginine•HCl0.084
L-Cystine0.048
L-GlutamineAbsent
Glycine0.03
L-Histidine•HCl•H2O0.042
L-Isoleucine0.1048
L-Leucine0.1048
L-Lysine•HCl0.1462
L-Methionine0.03
L-Phenylalanine0.066
L-Serine0.042
L-Threonine0.0952
L-Tryptophan0.016
L-Tyrosine0.072
L-Valine0.0936
Vitamins:
Choline Chloride0.004
Folic Acid0.004
i-Inositol0.0072
Nicotinamide0.004
D-Pantothenic Acid, Na0.004
Pyridoxal•HCl0.004
Riboflavin0.0004
Thiamine•HCl0.004
Other:
D-Glucose4.5
Phenol Red, Sodium0.0159
Pyruvic Acid, Sodium0.11
Total:12.66g/Liter (1X)
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 12.66g in 800-900ml of ddH2O stirring gently until completely solubilized. Do NOT heat. If required, add 3.7g sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light orange, homogenous, free flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R. and Freeman, G. Virology. 8, 396-397. (1959). 2. Smith, J.D., Freeman,G., Vogt, M. and Dulbecco, R. The Nucleic Acid of Polyoma Virus. 12, 185-196. (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89. (1970). 4. Rutzky, L.P. and Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro. 9, 468. (1974).