Technical Data
Media
Dulbecco’s MEM (DMEM) High Glucose, Low Na, K, Ca w/Pyridoxal•HCl (Powder)
D9803-05
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Note: Most Sodium, Potassium and Calcium salts have been eliminated.
Inorganic Salts:
Calcium Chloride•2H2OAbsent
Ferric Nitrate•9H2O0.0001
Magnesium Sulfate, Anhy0.09767
Potassium ChlorideAbsent
Sodium ChlorideAbsent
Sodium Phosph Mono•H2OAbsent
Amino Acids:
L-Arginine•HCl0.084
L-Cystine•2HCl0.0626
L-Glutamine0.584
Glycine0.030
L-Histidine•HCl•H2O0.042
L-Isoleucine0.105
L-Leucine0.105
L-Lysine•HCl0.146
L-Methionine0.030
L-Phenylalanine0.066
L-Serine0.042
L-Threonine0.095
L-Tryptophan0.016
L-Tyrosine•2Na•2H2O0.10379
L-Valine0.094
Vitamins:
Choline Chloride0.004
Folic Acid0.004
myo-Inositol0.0072
Niacinamide0.004
D-Pantothenic Acid•Ca0.002
Pyridoxal•HCl0.004
Riboflavin0.0004
Thiamine•HCl0.004
Other:
D-Glucose4.500
Phenol Red, Sodium0.0159
Pyruvic Acid, Sodium0.110
Total:6.359
Many modifications of Eagle’s Medium have been developed since the original formulation appeared in literature. Among the most widely used of these modifications is Dulbecco’s Modified Eagle’s Medium (DME). DME is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and viatamins, as well as additional supplementary components. The original DME formula contains 1000mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500mg/L glucose has proved to be optimal in cultivating certain cell types.


Directions per Liter: Dissolve 6.36g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light orange to pink, homogenous, free flowing powder

Solubility: Red, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).