Technical Data
Media
Dulbecco’s MEM (DMEM) High Glucose, w/ L-Glutamine, HEPES, Pyridoxal HCl w/o Na Pyruvate (Powder)
D9804
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O264.92
Ferric Nitrate•9H2O0.10
Potassium Chloride400
Magnesium Sulfate97.67
Sodium Chloride6400
Sodium Phosphate Monobasic108.69
Amino Acids:
L-Arginine•HCI84
L-Cystine•2HCI62.53
L-Glutamine584
Glycine30
L-Histidine•HCl•H2O42
L-Isoleucine104.8
L-Leucine104.8
L-Lysine•HCI146.2
L-Methionine30
L-Phenylalanine66
L-Serine42
L-Threonine95.2
L-Tryptophan16
L-Tyrosine•2Na•2H2O103.79
L-Valine93.6
Vitamins:
D-Pantothenic Acid•Ca4
Choline Chloride4
Folic Acid4
i-Inositol7
Nicotinamide4
Pyridoxal HCI4
Riboflavin0.4
Thiamine HCI4
Other:
D-Glucose4500
HEPES Free Acid5958
Phenol Red15.34
Total:19.381g/liter
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 19.38g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to off white, homogenous, free flowing powder

Solubility (19.4g/L): Yellow, clear, complete

pH: 5.8 ± 0.4

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).