Technical Data
Media
Dulbecco’s MEM (DMEM) F-12 w/o Glucose
D9807-02
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride 2H2O0.154
Cupric Sulfate•5H2O 0.0000013
Ferric Nitrate•9H2O0.00005
Ferrous Sulfate•7H2O0.000417
Magnesium Chloride 0.02866
Magnesium Sulfate0.04884
Potassium Chloride0.3118
Sodium Chloride6.996
Sodium Phosphate dibasic 0.07102
Sodium Phosphate Monobasic 0.0543
Zinc Sulfate•7H2O0.000432
Amino Acids:
L-Alanine0.00445
L-Arginine0.12197
L-Asparagine•H2O 0.0075
L-Aspartic Acid0.00665
L-Cysteine•HCl•H2O 0.01756
L-Cystine•2HCl 0.03129
L-Glutamic Acid 0.00735
L-Glutamine0.365
Glycine0.01875
L-Histidine HCl•H2O 0.03148
L-Isoleucine 0.05447
L-Leucine0.05905
L-Lysine•HCl0.09125
L-Methionine0.01724
L-Phenylalanine0.03548
L-Proline0.01725
L-Serine0.02625
L-Threonine0.05345
L-Tryptophan0.00902
L-Tyrosine•2Na•2H20 0.05579
L-Valine0.05285
Vitamins:
Biotin0.0000035
Choline Chloride0.00898
Vitamin B120.00068
Folic Acid0.00266
i-inositol0.0126
Nicotinamide0.00202
D-Pantothenic Acid•Ca0.00224
Pyridoxal Hydrochloride 0.002
Pyridoxine HCl 0.000031
Riboflavin0.000219
Thiamine HCl0.00217
Other:
Thymidine0.000365
D-Glucose Absent
Hypoxanthine0.0021
Linoleic Acid0.000042
Lipoic Acid0.000105
Phenol Red•Na0.00863
Putrescine•HCl 0.000081
Pyruvic Acid, Sodium0.055
Total:8.85g/Liter
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 8.85 grams in 900ml of ddH2O stirring gently until completely solubilized. DO NOT heat. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light orange to orange, homogenous, free flowing powder

Solubility: Light pink to orange, clear, complete

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).