Technical Data
Media
Dulbecco’s MEM (DMEM) F-12, Reh Modification w/o L-Aspartic Acid, L-Glutamic Acid
D9807-05
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride2H2O0.154
Cupric Sulfate•H2O0.0000013
Ferric Nitrate9H2O0.00005
Ferrous Sulfate•7H2O0.000417
Magnesium Chloride6H2O0.0612
Magnesium Sulfate0.04884
Potassium Chloride0.3118
Sodium Chloride6.996
Sodium Phosphate dibasic0.07102
Sodium Phosphate monobasic0.0625
Zinc Sulfate•7H2O0.000432
Amino Acids:
L-Alanine0.00445
L-Arginine•HCl0.1475
L-Asparagine•H2O0.0075
L-Aspartic AcidAbsent
L-Cysteine•HCl•H2O0.01756
L-Cystine•2HCl0.03129
L-Glutamic AcidAbsent
L-Glutamine0.365
Glycine0.01875
L-Histidine HCl•H2O0.03148
L-Isoleucine0.05447
L-Leucine0.05905
L-Lysine•HCl0.09125
L-Methionine0.01724
L-Phenylalanine0.03548
L-Proline0.01725
L-Serine0.02625
L-Threonine0.05345
L-Tryptophan0.00902
L-Tyrosine•2Na•2H200.05579
L-Valine0.05285
Vitamins:
Biotin0.0000035
Choline Chloride0.00898
VitaminB120.00068
Folic Acid0.00265
i-inositol0.0126
Niacinamide0.00202
D-Pantothenic Acid•Ca0.00224
Pyridoxal Hydrochloride0.002
Pyridoxine HCl0.000031
Riboflavin0.000219
Thiamine HCl0.00217
Other:
Thymidine0.000365
D-Glucose3.151
HEPES3.5745
Hypoxanthine0.00239
Linoleic Acid0.000042
Lipoic Acid0.000105
Phenol Red0.0081
Putrescine•HCl0.000081
Pyruvic Acid,Sodium0.055
Total:15.627g/Liter
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 15.63g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light tan to light orange, homogeneous, free flowing powder.

Solubility: Pink to orange, clear, complete

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).