Technical Data
Media
Dulbecco’s MEM (DMEM) F-12 w/o Vitamins, Linoleic Acid, Lipoic Acid, Putrescine
D9807-07
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Calcium Chloride 2H2O0.154
Cupric Sulfate•H2O 0.0000013
Ferric Nitrate 9H2O0.00005
Ferrous Sulfate•7H2O0.000417
Magnesium Chloride 6H2O0.0612
Magnesium Sulfate0.04884
Potassium Chloride0.3118
Sodium Chloride6.996
Sodium Phosphate dibasic 0.07102
Sodium Phosphate monobasic 0.0543
Zinc Sulfate•7H2O0.000432
L-Alanine0.00445
L-Arginine•HCl0.1475
L-Asparagine•H2O 0.0075
L-Aspartic Acid0.00665
L-Cysteine•HCl•H2O 0.01756
L-Cystine•2HCl0.03129
L-Glutamic Acid 0.00735
L-Glutamine0.365
Glycine0.01875
L-Histidine HCl•H2O 0.03148
L-Isoleucine 0.05447
L-Leucine0.05905
L-Lysine•HCl0.09125
L-Methionine0.01724
L-Phenylalanine0.03548
L-Proline0.01725
L-Serine0.02625
L-Threonine0.05345
L-Tryptophan0.00902
L-Tyrosine•2Na•2H20 0.05579
L-Valine0.05285
Biotinabsent
Choline Chlorideabsent
Vitamin B12absent
Folic Acidabsent
i-inositolabsent
Nicotinamideabsent
D-Pantothenic Acid•Caabsent
Pyridoxal Hydrochloride absent
Pyridoxine HCl absent
Riboflavinabsent
Thiamine HClabsent
Thymidine0.000365
D-Glucose3.15
Hypoxanthine•Na0.0021
Linoleic Acidabsent
Lipoic Acidabsent
Phenol Red, Sodium0.0081
Putrescine•HCl absent
Pyruvic Acid, Sodium0.055
Total: 12.02g/L
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 12.06g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light orange, homogeneous, free flowing powder. Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).