Technical Data
Media
Dulbecco’s MEM (DMEM) F-12 w/HEPES, Sodium Bicarbonate w/o Amino Acids, Pyridoxal Hydrochloride
D9807-09
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride 2H2O0.154
Cupric Sulfate•H2O 0.0000013
Ferric Nitrate 9H2O0.00005
Ferrous Sulfate•7H2O0.000417
Magnesium Chloride 6H2O0.0612
Magnesium Sulfate0.04884
Potassium Chloride0.3118
Sodium Bicarbonate1.2
Sodium Chloride6.996
Sodium Phosphate dibasic 0.07102
Sodium Phosphate monobasic 0.0625
Zinc Sulfate•7H2O0.000432
Amino Acids:
L-AlanineAbsent
L-Arginine•HClAbsent
L-Asparagine•H2O Absent
L-Aspartic AcidAbsent
L-Cysteine•HCl•H2O Absent
L-Cystine•2HClAbsent
L-Glutamic Acid Absent
L-GlutamineAbsent
GlycineAbsent
L-Histidine HCl•H2O Absent
L-Isoleucine Absent
L-LeucineAbsent
L-Lysine•HClAbsent
L-MethionineAbsent
L-PhenylalanineAbsent
L-ProlineAbsent
L-SerineAbsent
L-ThreonineAbsent
L-TryptophanAbsent
L-Tyrosine•2Na•2H20 Absent
L-ValineAbsent
Vitamins:
Biotin0.0000035
Choline Chloride0.00898
Vitamin B120.00068
Folic Acid0.00266
i-inositol0.0126
Nicotinamide0.00202
D-Pantothenic Acid•Ca0.00224
Pyridoxal Hydrochloride Absent
Pyridoxine HCl 0.002
Riboflavin0.000219
Thiamine HCl0.00217
Other:
Thymidine0.000365
D-Glucose3.15
HEPES3.57
Hypoxanthine (Na)0.00239
Linoleic Acid0.000042
Lipoic Acid0.000105
Phenol Red0.0081
Putrescine•HCl 0.000081
Pyruvic Acid, Sodium0.055
Total:15.73g/Liter
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter: Dissolve 15.73g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light orange, homogeneous, free flowing powder.

Solubility: Orange to red, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).