Technical Data
Media
Dulbecco’s MEM (DMEM) Ham’s F-12 for SFM, Ready-to-use with L-glutamine, Hepes, BPE and EGF
D9811-02B
Cell Culture Grade
Storage -70°C    Shipping Dry Ice
Components shown as mg/liter
Inorganic Salts:mg/L
Calcium Chloride, anhy116.65
Cupric Sulfate0.0008
Ferric Nitrate•9H2O0.050
Ferrous Sulfate•7H2O0.417
Potassium Chloride311.8
Magnesium Sulfate84.95
Sodium Chloride7000.0
Sodium Phosphate monobasic•H2O62.50
Sodium Phosphate dibasic 71.00
Zinc Sulfate•7H2O0.432
Amino Acids:
L-Alanine4.45
L-Arginine•HCl147.50
L-Asparagine•H2O 7.50
L-Aspartic Acid6.65
L-Cysteine•HCI•H2O 17.56
L-Cystine•2HCl31.29
L-Glutamic Acid 7.35
Glycine18.75
L-Histidine HCl•H2O 31.48
L-Isoleucine 54.37
L-Leucine58.95
L-Lysine•HCI91.35
L-Methionine17.24
L-Phenylalanine35.48
L-Proline17.25
L-Serine26.25
L-Threonine53.55
L-Tryptophan9.02
L-Tyrosine•Na2•2H2O55.82
L-Valine52.85
Vitamins:
Biotin0.0037
D-Pantothenic Acid•Ca2.24
Choline Chloride8.98
Folic Acid2.65
i-Inositol12.61
Nicotinamide2.019
Pyridoxine HCI 2.031
Riboflavin0.219
Thiamine HCI2.169
Vitamin B120.68
Other:
D-Glucose3151.0
Hypoxanthine•Na2.385
Methyl Linoleate0.044
Lipoic Acid0.105
Phenol Red•Na8.10
Putrescine•2HCl 0.08
Sodium Pyruvate 110.00
Thymidine0.365
Total:11.7g/L
DMEM F/12
Serum-Free Media

Ready-to-use with L-glutamine, Hepes, BPE and EGF
Designed to culture wide range of cells
Complete serum-free medium
Antibiotic-free

DMEM/F12 is a serum-free medium formulation for general use. This formulation is a 1:1 blend of DMEM and Ham's F12 media supplied complete, ready to use with L-glutamine, Hepes, BPE and EGF for culturing a wide range of cell types. DMEM/F12 does not contain phenol red or antibiotics.

AthenaES™ also offers custom formulation of DMEM and other cell culture media. To request a custom tissue culture media quote or place an order, call us at 888-892-8408 or Click Here to submit an online form.

Storage Store at -80°C, supplied frozen
Stability at 4°C 4-6 weeks
pH 7.3 ± 0.2
Osmolality 270-300 mOsM


Directions per Liter Dissolve 11.7 grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjusting pH as necessary. If required, add sodium bicarbonate with stirring when solution has cooled. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Barnes, D., Sato, G. Analytical Biochemistry 102: 255-270 (1980). 2. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 3. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus 12: 185-196 (1960). 4. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6: 89 (1970). 5. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media. In Vitro 9: 468 (1974).