Dulbecco’s MEM (DMEM) Ham’s F-12 for SFM, Ready-to-use with L-glutamine, Hepes, BPE and EGF
Cell Culture Grade
Storage -70°C Shipping Dry Ice
Components shown as mg/liter
Ready-to-use with L-glutamine, Hepes, BPE and EGF
Designed to culture wide range of cells
Complete serum-free medium
DMEM/F12 is a serum-free medium formulation for general use. This formulation is a 1:1 blend of DMEM and Ham's F12 media supplied complete, ready to use with L-glutamine, Hepes, BPE and EGF for culturing a wide range of cell types. DMEM/F12 does not contain phenol red or antibiotics.
AthenaES™ also offers custom formulation of DMEM and other cell culture media. To request a custom tissue culture media quote or place an order, call us at 888-892-8408 or Click Here to submit an online form.
Storage Store at -80°C, supplied frozen
Stability at 4°C 4-6 weeks
pH 7.3 ± 0.2
Osmolality 270-300 mOsM
Directions per Liter Dissolve 11.7 grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjusting pH as necessary. If required, add sodium bicarbonate with stirring when solution has cooled. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Barnes, D., Sato, G. Analytical Biochemistry 102: 255-270 (1980). 2. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 3. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus 12: 185-196 (1960). 4. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6: 89 (1970). 5. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media. In Vitro 9: 468 (1974).