Technical Data
Media
Dulbecco’s MEM (DMEM) Ham’s F-12, 1:1 w/15mM HEPES, Low Glucose w/o Choline, Glutamine, Methionine, Pyruvate (Powder)
D9811-03
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride116.00
Cupric Sulfate•5H2O0.0013
Ferric Nitrate•9H2O0.05
Ferrous Sulfate•7H2O0.417
Magnesium Chloride28.64
Magnesium Sulfate48.84
Potassium Chloride311.80
Sodium Chloride6995.5
Sodium Phosphate Dibasic71.02
Sodium Phosphate Monobasic62.50
Zinc Sulfate•7H2O0.432
Amino Acids:
L-Alanine4.45
L-Arginine•HCl147.5
L-Asparagine•H2O7.50
L-Aspartic Acid6.65
L-Cysteine HCl•H2O17.56
L-Cystine•2HCI31.29
L-Glutamic Acid7.35
L-GlutamineAbsent
Glycine18.75
L-Histidine HCl•H2O31.48
L-Isoleucine54.47
L-Leucine59.05
L-Lysine•HCI91.25
L-MethionineAbsent
L-Phenylalanine35.48
L-Proline17.25
L-Serine26.25
L-Threonine53.45
L-Tryptophan9.02
L-Tyrosine•2Na•2H2055.79
L-Valine52.85
Vitamins:
Biotin0.0035
Vitamin B120.68
Choline ChlorideAbsent
Folic Acid2.65
i-Inositol12.60
Nicotinamide2.02
D-Pantothenic Acid•Ca2.24
Pyridoxal HCI2
Pyridoxine HCI2.031
Riboflavin0.219
Thiamine HCI2.17
Other:
Thymidine0.365
D-Glucose1000
HEPES Free Acid3574.5
Hypoxanthine, Sodium2.39
Linoleic Acid0.042
Lipoic Acid0.105
Phenol Red8.305
Putrescine•2HCl0.081
Sodium PyruvateAbsent
Total:12.975g/L
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham’s mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells.


Directions per Liter: Dissolve 12.975g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light orange to orange, homogeneous, free flowing powder. Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Barnes, D., Sato, G. Analytical Biochemistry 102: 255-270 (1980). 2. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 3. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus 12: 185-196 (1960). 4. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6: 89 (1970). 5. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media. In Vitro 9: 468 (1974).