Technical Data
Media
Dulbecco’s MEM (DMEM) Ham’s F-12 w/o Calcium, Phenol Red, w/ Ferric Nitrate (Powder)
D9811-14B
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride DihydrateAbsent
Cupric Sulfate•5H2O0.0013
Ferric Nitrate•9H2O0.05
Ferrous Sulfate•7H2O0.417
Potassium Chloride311.8
Magnesium Chloride28.66
Magnesium Sulfate48.84
Sodium Chloride6996
Sodium Phosphate Monobasic54.3
Sodium Phosphate Dibasic71.0
Zinc Sulfate•7H2O0.432
Amino Acids:
L-Alanine4.45
L-Arginine121.97
L-Asparagine•H2O7.5
L-Aspartic Acid6.65
L-Cysteine•HCI•H2O17.56
L-Cystine•2HCI31.29
L-Glutamic Acid7.35
L-Glutamine365
Glycine18.75
L-Histidine HCl•H2O31.48
L-Isoleucine54.47
L-Leucine59.05
L-Lysine•HCI91.25
L-Methionine17.24
L-Phenylalanine35.48
L-Proline17.25
L-Serine26.25
L-Threonine53.45
L-Tryptophan9.02
L-Tyrosine55.79
L-Valine52.85
Vitamins:
Biotin0.0035
D-Pantothenic Acid•Na2.24
Choline Chloride8.98
Folic Acid2.66
i-inositol12.60
Nicotinamide2.02
Pyridoxal HCI2.00
Pyridoxine HCI0.031
Riboflavin0.219
Thiamine HCI2.17
Cyanocobalamin (Vitamin B12)0.680
Other:
D-Glucose3150
Hypoxanthine2.1
Linoleic Acid0.042
Lipoic Acid0.105
Putrescine•HCl0.081
Sodium Pyruvate55.05
Thymidine0.365
Phenol RedAbsent
Total:11.84g/L
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham’s mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells.


Directions per Liter: Dissolve 11.84g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white, homogeneous, free flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Barnes, D., Sato, G. Analytical Biochemistry 102: 255-270 (1980). 2. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 3. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus 12: 185-196 (1960). 4. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6: 89 (1970). 5. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media. In Vitro 9: 468 (1974). 6. Kaighn, M.E., (1973) in “Tissue Culture Methods and Applications,” Kruse, Jr., 7. P.F. and Patterson, Jr., M.D., eds., Academy Press, NY, 54-58. 8. Robey, P.G. and Termine, J.D. (1985) Calcif. Tissue Int. 37:453-460.