Technical Data
Media
Dulbecco’s MEM (DMEM) Ham’s F-12 w/o Calcium Chloride, Phenol Red, Cystine, Methyl Linoleate (Powder)
D9811-14C
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride DihydrateAbsent
Cupric Sulfate•5H2O0.002
Ferrous Sulfate•7H2O0.80
Magnesium Chloride•6H2O106
Magnesium Sulfate•7H2O393
Potassium Chloride285
Potassium Phosphate Monobasic59
Sodium Chloride7530
Sodium Phosphate Dibasic•7H2O218
Zinc Sulfate•7H2O0.14
Amino Acids:
L-Alanine17.80
L-Arginine•HCl421.30
L-Asparagine•H2O30.00
L-Aspartic Acid26.60
L-Cysteine•HCI•H2O70.04
L-CystineAbsent
L-Glutamic Acid29.40
Glycine15.00
L-Histidine HCl•H2O41.90
L-Isoleucine7.90
L-Leucine26.20
L-Lysine•HCI73.10
L-Methionine8.90
L-Phenylalanine9.90
L-Proline69.10
L-Serine21.00
L-Threonine23.80
L-Tryptophan4.10
L-Tyrosine10.90
L-Valine23.40
Vitamins:
Biotin0.07
Choline Chloride13.96
Cyanocobalamin (Vitamin B12)1.36
Folic Acid1.32
i-inositol18.00
Nicotinamide0.04
D-Pantothenic Acid•Ca0.48
Pyridoxine HCI0.06
Riboflavin0.04
Thiamine HCI0.21
Other:
Thymidine0.70
D-Glucose4500.00
Hypoxanthine4.00
DL-Thioctic Acid0.21
Methyl LinoleateAbsent
Phenol RedAbsent
Putrescine•HCl0.30
Sodium Pyruvate220.00
Total:14.28g/L
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham’s mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells.


Directions per Liter: Dissolve 14.28g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to off-white, homogeneous, free flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Barnes, D., Sato, G. Analytical Biochemistry 102: 255-270 (1980). 2. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 3. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus 12: 185-196 (1960). 4. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6: 89 (1970). 5. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media. In Vitro 9: 468 (1974). 6. Kaighn, M.E., (1973) in “Tissue Culture Methods and Applications,” Kruse, Jr., 7. P.F. and Patterson, Jr., M.D., eds., Academy Press, NY, 54-58. 8. Robey, P.G. and Termine, J.D. (1985) Calcif. Tissue Int. 37:453-460